sinI [2019-09-08 17:09:27]

antagonist of [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR], drives [protein|920F91E748EE079FF864011D9052B073567C41E4|SlrR] from the [protein|920F91E748EE079FF864011D9052B073567C41E4|SlrR](LOW) to the [protein|920F91E748EE079FF864011D9052B073567C41E4|SlrR](HIGH) state

locus: BSU_24600

pI: 6.33

mw: 6.47 kDa

protein length: 57 aa

gene length: 174 bp

function: control of [SW|biofilm formation]

product: antagonist of [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.6|Transition state regulators]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.4|Regulation]

Gene

Phenotypes of a mutant

• altered cell death pattern in colonies [Pubmed|23012477]

The protein

Paralogous protein(s)

• [protein|C17C296F3EB2E106C380E3B5D784F3FA63F4C9B7|SlrA]

[SW|Domains]

• [SW|Sin domain] (aa 2-40) (according to UniProt)

Structure

• [PDB|1B0N] (complex [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]-[protein|24D6A7DDCB25B99FBB670E9203F826596916B35C|SinI]) [Pubmed|9799632]

Expression and Regulation

Operons

• genes: [gene|24D6A7DDCB25B99FBB670E9203F826596916B35C|sinI]-[gene|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]
  description: [Pubmed|3125149]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|3125149], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, in a portion of cells [Pubmed|11751836], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, [Pubmed|1664536], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [PubMed|7635837,11751836], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC]: repression, [PubMed|1906467,11751836], in [regulon|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC regulon]

  regulation

  • repressed during exponential growth ([protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1906467,11751836 PubMed]

  additional information

  • the mRNA is stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• GP959 (spc), available in [SW|Jörg Stülke]'s lab
• DS91 (spc) NCIB3610 derivate, available in [SW|Jörg Stülke]'s lab
• 1S98 ( ''sinI''::''kan''), [Pubmed|11466285], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S98&Search=1S98 BGSC]
• GP1663 (''[gene|22BEB39F735DAAF71F1B382CD453A9C2D4CC9FA4|yqhG]-[gene|24D6A7DDCB25B99FBB670E9203F826596916B35C|sinI]-[gene|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]-[gene|BF97457E986656E4A9FE7A858F5BDF1759850D5C|tasA]''), available in [SW|Jörg Stülke]'s lab
• BKE24600 ([gene|24D6A7DDCB25B99FBB670E9203F826596916B35C|sinI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCAGTTTCTCCTCCTAA, downstream forward: _UP4_TGAATGTGCTATAATATCAC
• BKK24600 ([gene|24D6A7DDCB25B99FBB670E9203F826596916B35C|sinI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCAGTTTCTCCTCCTAA, downstream forward: _UP4_TGAATGTGCTATAATATCAC

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

References

Reviews

• 20541494

Modelling of the [protein|24D6A7DDCB25B99FBB670E9203F826596916B35C|SinI]]/[[SinR] switch

• 21095906

Original publications

• 23430750,23012477,21326214,21815947,3125149,15661000,18047568,11751836,1906467,11751836,7635837,11751836,10547280,15104138,9799632,20923420,8422983,24256735